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目的 观察N -已酰半胱氨酸 (N -Acetylcysteine ,NAC)对离体大鼠肠系膜动脉内皮依赖性血管舒张作用以及通过增加一氧化氮 (nitricoxide ,NO)的活性达到扩张血管的作用。方法 采用离体大鼠肠系膜动脉血管对化学药物刺激所产生的收缩与舒张变化 ,用U4 6619作为收缩后的基础长度值 ,加入药物后的长度变化与基础长度比较 ,可以得出舒张百分比 ,以了解药物的舒张血管作用的强度和程度。结果 加入NAC(浓度 5、10、15、2 0、2 5、30mmol/L) ,没有直接的血管舒张作用 ;NAC +ACh(NAC浓度每点均为 30mmol/L ,ACh浓度为 1、10、10 0、10 0 0、10 0 0 0、10 0 0 0 0nmol/L)的血管舒张作用为 94% ,而单纯加入ACh(浓度同前 )的舒张作用仅为 6 4% ,统计学分析P <0 0 1;NAC +A2 3 187(NAC浓度每点为 30mmol/L ,A2 3 187浓度为 1、10、10 0、10 0 0、10 0 0 0、10 0 0 0 0nmol/L)血管舒张作用为 92 % ,而单纯加入A2 3 187(浓度同前 )的舒张作用仅为 6 8% ,统计学分析P <0 0 1。结论 NAC没有直接的血管扩张作用 ,但它可以通过增加内皮依赖性血管扩张剂的作用 ,激活NO受体 ,从而达到较强的扩张血管的目的 ,为临床工作提供了有利的理论基础。
Objective To observe the effect of N-acylcysteine (NAC) on the endothelium-dependent vasodilatation of isolated rat mesenteric artery and the dilation of blood vessels by increasing the activity of nitric oxide (NO). Methods The contraction and relaxation of chemotherapeutic drugs induced by isolated rat mesenteric artery were compared. U4 6619 was used as the basic length after contraction, and the change of length after drug addition was compared with the base length to get the percentage of diastole. Understand the strength and extent of the drug’s vasodilatation. Results NAC + ACh (NAC concentration was 30mmol / L, ACh concentration was 1, 10, The vasorelaxation effect of ACh (concentration was the same as before) was only 64%. The statistical analysis of P <0 0 1; NAC + A2 3 187 (NAC concentration 30 mmol / L per point, A2 3 187 concentration 1,10,10 0,10 0 0,10 0 0 0,10 0 0 0 0 nmol / L) The diastolic effect was 92%, while only adding A2 3 187 (with the same concentration) diastolic effect was only 68%, P <0.01. Conclusion NAC has no direct vasodilator effect, but it can activate NO receptor by increasing the role of endothelium-dependent vasodilator so as to achieve the purpose of dilating blood vessels. It provides a good theoretical basis for clinical work.