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目的探讨中药清肺化痰丸对大气PM2.5致大鼠肺损伤的干预作用。方法于某居住区采集大气PM2.5。健康成年SD雄性大鼠64只随机分为8组:每组8只,分别为空白对照组(不做任何处理)、生理盐水对照组(气管滴注1.5 ml/kg生理盐水)、溶剂对照组(每天灌胃给予10 ml/kg的0.2%羧甲基纤维素,共1周)、干预对照组(每天灌胃给予2.40 g/kg清肺化痰丸,共1周)、染毒对照组(气管滴注40 mg/kg的PM2.5生理盐水混悬液染毒,隔天1次,共3次)以及低、中、高剂量干预组(气管滴注40 mg/kg的PM2.5生理盐水混悬液染毒,隔天1次,共3次,再每天分别灌胃给予0.65、1.20和2.40 g/kg清肺化痰丸,共1周。收集肺泡灌洗液(BALF)进行细胞计数,并测定BALF中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、乳酸脱氢酶(LDH)、总蛋白(TP)、白蛋白(ALB)、白介素-1β(IL-1β)、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)水平。结果与生理盐水对照组比较,染毒对照组大鼠BALF中ACP、AKP、LDH活力和TP、ALB、IL-6、IL-1β、TNF-α含量及中性粒细胞百分比均升高,差异均有统计学意义(P<0.05)。与染毒对照组比较,各剂量干预组大鼠BALF中ACP、AKP、LDH活力和TP、ALB、IL-6、IL-1β、TNF-α含量及中性粒细胞百分比均降低,差异均有统计学意义(P<0.05),且随干预剂量的升高其降低幅度增加。结论中药清肺化痰丸对气管滴注PM2.5导致的肺损伤有一定减轻作用。
Objective To investigate the effect of Qingfei Huatan Pills on lung injury induced by atmospheric PM2.5 in rats. Methods Collect atmospheric PM2.5 in a residential area. Sixty-four healthy SD male rats were randomly divided into 8 groups: 8 in each group, which were blank control group (without any treatment), saline control group (tracheal instillation 1.5 ml / kg saline), solvent control group (Daily administration of 0.2% carboxymethylcellulose at 10 ml / kg for 1 week), and the control group (2.40 g / kg Qingfei Huatan Pills given orally daily for 1 week), the control group (Tracheal instillation of 40 mg / kg PM2.5 saline suspension once a day for 3 times), and low, middle and high dose intervention groups (PM2.5 tracheal instillation 40 mg / kg Saline suspension, once a day for 3 times, and then given 0.65, 1.20 and 2.40 g / kg Qingfei Huatan Pills by gavage daily for 1 week.The bronchoalveolar lavage fluid (BALF) was collected for The cell counts and BALF determination of acid phosphatase (ACP), alkaline phosphatase (AKP), lactate dehydrogenase (LDH), total protein (TP), albumin (ALB), interleukin- , Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) .Results Compared with saline control group, the activities of ACP, AKP, LDH and the activities of TP, ALB, IL -6, IL-1β, TNF-α and neutrophil percentage were increased (P <0.05) .Compared with the control group, the activities of ACP, AKP, LDH and the activities of TP, ALB, IL-6, IL- α content and neutrophil percentage were decreased, the difference was statistically significant (P <0.05), and with the increase of intervention dose, the decreasing extent increased.Conclusion PM2.5 tracheal instillation caused by Chinese herbs Qingfei Huatan Pill The lung injury has some relief.