虽然用基因工程方法在低等真核细胞中超表达异源蛋白已取得很大进展,但许多用于治疗的需转译后加工的蛋白质只能在哺乳动物细胞中生产。此类细胞在体外培养除要求基本营养物质外,还需要一系列生长因子,一般以动物血清或动物蛋白的形式提供。这种化学成分不明的混合物常造成批与批间组 Although much progress has been made in the genetic engineering of overexpression of heterologous proteins in lower eukaryotic cells, many of the proteins that are required for post-translational processing for treatment can only be produced in mammalian cells. In vitro culture of such cells, in addition to requiring essential nutrients, also requires a range of growth factors, usually provided in the form of animal serum or animal protein. This mixture of unknown chemical composition often results in lots and lots