既往认为,仅痢疾志贺氏菌1型能产生不同于内毒素的神经毒素。本文证实了近年来 Keusch 等的新发现:福氏志贺氏菌2a 型也可产生此类毒素。作者将痢疾志贺氏菌1型60R 株(粗糙型变种)和福氏志贺氏菌2a 型 M4243株(毒力株)培养在改良的 Syncase 肉汤中,收集菌细胞经加热灭活后用硷提取。结果发现在痢疾志贺氏菌1型的菌细胞的硷提取物、无菌肉汤培养滤液以及声波击碎菌细胞的无菌滤液中均有毒素。而福氏志贺氏菌的硷提取 Historically, only Shigella dysenteriae Type 1 can produce a neurotoxin different from endotoxin. This article confirms the recent discovery by Keusch et al. That Shigella flexneri 2a also produces such toxins. The authors cultivated Shigella dysenteriae type 1 60R (rough variant) and Shigella flexneri 2a type M4243 (virulent strain) in a modified Syncase broth, the cells were harvested by heat inactivation Alkali extraction. As a result, it was found that toxins were present in the alkaline extract of Shigella dysenteriae type 1 bacterial cells, the filtrate of the aseptic broth culture, and the sterile filtrate of the sonicated cells. And Shigella flexneri alkaline extraction