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目的探讨三七总皂苷能否抑制急性坏死性胰腺炎(ANP)大鼠肺核转录因子-κB(NF-κB)的活性,并减轻ANP时肺组织病理损害。方法30只SD大鼠被随机分为假手术组(sham组)、ANP模型组和三七总皂苷预处理组,每组10只。sham组和ANP模型组制模前1 h腹腔注射质量分数为0.9%的生理盐水(1 ml/kg),三七总皂苷组制模前1 h腹腔注射50 g/L三七总皂苷(1 ml/kg)。用质量分数为5%的牛磺胆酸钠经大鼠胰胆管逆行注射制备大鼠ANP模型;sham组进腹后仅翻动胰腺和十二指肠后关腹,不注入牛磺胆酸钠。各组于制模6 h后处死大鼠,取肺组织测定湿/干重(W/D)比值和NF-κB活性。结果ANP模型组肺组织NF-κB阳性细胞率70%~100%所占比例(7只比0只)和阳性细胞数〔(72.52±7.63)PU比(33.09±4.75)PU〕明显高于sham组(P均<0.05),经三七总皂苷预处理后NF-κB阳性细胞率70%~100%所占比例(0只比7只)和阳性细胞数〔(42.77±9.79)PU比(72.52±7.63)PU〕较ANP模型组明显下降(P均<0.05)。ANP模型组肺组织病理评分及W/D比值均较sham组显著增高,而三七总皂苷组较ANP模型组明显改善(P<0.05或P<0.01)。结论NF-κB在ANP大鼠肺组织中被明显激活;三七总皂苷可以在体内抑制肺组织中NF-κB的激活,减轻各脏器的病理损害。
Objective To investigate whether Panax notoginseng saponins can inhibit the activity of nuclear factor-κB (NF-κB) in rats with acute necrotizing pancreatitis (ANP) and reduce the pathological damage of lung tissue during ANP. Methods Thirty SD rats were randomly divided into sham operation group (a sham group), ANP model group, and panax notoginseng saponin pretreatment group, 10 in each group. In the sham group and the ANP model group, normal saline 0.9% (1 ml/kg) was intraperitoneally injected 1 hour before the model preparation, and 50 g/L panax notoginseng saponin was intraperitoneally injected 1 hour before the preparation of the panax notoginseng saponins (1) Ml/kg). The rat ANP model was prepared by retrograde injection of sodium taurocholate in rat pancreaticobiliary duct with a mass fraction of 5%; the sham group only turned the pancreas and the duodenum and closed the abdomen after inflamed and did not inject sodium taurocholate. Rats were sacrificed 6 h after molding. Lung tissue was measured for wet/dry weight (W/D) ratio and NF-κB activity. Results The percentage of NF-κB positive cells in the lung tissue in the ANP model group was significantly higher than that in sham (70% to 00%) and the number of positive cells ((72.52±7.63)PU ratio (33.09±4.75)PU). In the group (P <0.05), the percentage of NF-κB positive cells after pretreatment with Panax notoginseng saponins was 70% to 100% (0 for 7) and the number of positive cells (42.77 ± 9.79) PU ratio ( 72.52±7.63)PU] was significantly decreased compared with ANP model group (P all < 0.05). Lung pathological scores and W/D ratios in the ANP model group were significantly higher than those in the sham group, while those in the Panax notoginseng saponins group were significantly better than those in the ANP model group (P<0.05 or P<0.01). Conclusion NF-κB is significantly activated in the lung tissue of ANP rats. Panax notoginseng saponins can inhibit the activation of NF-κB in lung tissue and reduce the pathological damage of various organs.