马铃薯脱毒苗主要是通过茎尖培养和病毒鉴定后获得的。要把少量的脱毒苗用到生产上去,必须扩大繁殖。通用的方法是把一株脱毒苗的每个叶片带茎切成一小段,放在培养基中,数日之后在叶腋处萌芽,约20天左右即可形成一株脱毒苗。这就是通常所说的“切段繁殖”。采用的培养基有MA、MS、White、N_6等。国内多用固体培养,国 Virus-free potato seedlings are mainly obtained through shoot tip culture and virus identification. To a small amount of virus-free vaccine used in the production, we must expand breeding. The common method is to cut a leaf of each leaf of a virus-free seedling into small pieces and place it in culture medium. After a few days germinate in leaf axils and form a virus-free seedling in about 20 days. This is what is commonly known as “segmented reproduction.” The medium used are MA, MS, White, N_6 and so on. Domestic use of solid culture, the country