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以对白叶枯病高抗的水稻品种余水糯和高感的水稻品种浙辐802为材料,于2叶期用Tiron、Paraquat(PQ)和稻白叶枯病菌弱毒每株75-l进行诱导处理,测定诱导处理后3、24、48、72h叶中超氧化物歧化酶(SoD)活性、o2产生速率及丙二醛(MDA)含量的动态变化,并于3叶期用白叶枯病菌强毒株76-25进行挑战接种,结果表明:75-1和PQ均诱导两品种产生了系统抗性,并使诱导叶中o2产生速率和MDA含量增加。75-1使o2产生速率在24h达峰值,比对照升高3o.6%(余水糯)和25.3%(浙辐8o2);而PQ使o2产生速率在24~72h维持较高水平,比对照升高49.4%(余水糯)和39.7%(浙辐8o2)。MDA含量的变化晚于o2产生速率的改变,在72h达最高值。75-1使SoD活性降低,并在24h达最大降幅;而PQ使SoD活性升高,并在48h达峰值。Tiron使PQ和75-l的诱导效果减弱,并使75-l对余水糯和浙辐8o2的互作由非亲和性互作转变为类似亲和性互作,导致侵染斑的形成。
In this study, we used two primers, Tiron, Paraquat (PQ) and attenuated strain of rice leaf blight, were induced at a rate of 75-l per plant The activities of superoxide dismutase (SOD), the production rate of o2 and the content of malondialdehyde (MDA) in leaves at 3, 24, 48 and 72 h after induction treatment were determined. Strain 76-25 was challenged and inoculated. The results showed that both 75-1 and PQ induced systemic resistance in both cultivars and increased the o2 production rate and MDA content in induced leaves. 75-1 increased the o2 production rate to peak at 24 h, which was 3o higher than the control. 6% (Yu Shui waxy) and 25.3% (Zhefu 8o2) respectively. However, PQ increased the production rate of o2 from 24 to 72 hours and increased 49.4% (Yu Shui waxy) and 39.7% 8o2). The change of MDA content was later than that of o2, reaching the highest value at 72h. 75-1 decreased SoD activity and reached its maximum at 24 h, whereas PQ increased SoD activity and peaked at 48 h. Tiron reduced the inducing effect of PQ and 75-l, and changed the interaction of 75-1 on Yuwouuo and Zhefu 8o2 from non-affinity interaction to affinity-like interaction, resulting in the formation of infestation plaques .