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细胞的大规模培养是利用昆虫细胞培养生产杆状病毒的一个关键步骤。相对其他昆虫细胞培养系统来说,气升式反应器是一个较理想的选择。为了寻找适合细胞生长的反应器最优的结构参数,棉铃虫细胞HzAm1分别在不同几何参数的反应器中生长。下降室和上升室的截面积比,底部系数,高径比这些几何参数和表观气速都与细胞的生长紧密相关。在一定反应器尺寸下测定表观气速的影响,寻找一个能够提供足够氧分压而又不会对活细胞造成伤害的表观气速。并且,在不同尺寸反应器中表观气速与液体流速的关系能够测出来。改变反应器的上升室和下降室的截面积比和表观气速,活细胞的浓度可以从4.5×10~5提高到7.7×10~5cells//ml,细胞存活率从95.14%提高到98.38%。
Large-scale cell culture is a key step in the production of baculoviruses using insect cell culture. Airlift reactors are a better choice than other insect cell culture systems. In order to find the optimal structural parameters of the reactor suitable for cell growth, the cotton bollworm cells HzAm1 were respectively grown in reactors of different geometric parameters. The ratio of cross-sectional area, bottom coefficient and height-diameter ratio between the descending chamber and the ascending chamber are closely related to the cell growth. The effect of superficial gas velocity was measured at a given reactor size looking for an apparent gas velocity that provided sufficient oxygen partial pressure without damaging viable cells. Also, the relationship between superficial gas velocity and liquid flow rate in different size reactors can be measured. By changing the cross-sectional area ratio and superficial gas velocity of the ascending and descending chambers of the reactor, the viable cell concentration can be increased from 4.5 × 10-5 to 7.7 × 10-5 cells / ml and the cell viability increased from 95.14% to 98.38 %.