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本研究系统分析了大豆(品种:‘鲁豆4’)接种AM真菌Glomusfasciculatum和胞囊线虫(SCN,Heteroderaglycines)4号生理小种后各处理菌根和线虫侵染率、几丁质酶和苯丙氨酸解氨酶(PAL)活性及几丁质酶基因Chib1和苯丙氨酸解氨酶基因PAL5转录物的动态变化。结果表明,接种SCN对AM真菌的侵染率没有产生显著影响,但先接种AM真菌后接种SCN的大豆根内线虫侵染率明显低于只接种SCN的处理。另外,先接种AM真菌后接种SCN的大豆根内几丁质酶和PAL活性显著提高,活性高峰出现在接种线虫后的第3天。值得注意的是,先接种AM真菌后接种SCN的大豆根内两种基因Chib1和PAL5转录物高峰也出现在接种SCN后的第3天,即AM真菌侵染率快速上升而SCN侵染率快速下降时期。所以Chib1和PAL5基因的表达可能是AM真菌诱导的抗大豆胞囊线虫病害防御反应的一种表现。因此推测Chib1和PAL5直接参与了AM真菌诱导大豆抗胞囊线虫病害的防御反应。
In this study, we analyzed the infection rates of mycorrhizal and nematode in chickens (varieties: ’Lu Dou 4’) after inoculation with AM fungi Glomusfasciculatum and SCN (Heteroderalycines) Alanine aminotransferase (PAL) activity and the dynamic changes of chitinase gene chib1 and phenylalanine ammonia lyase gene PAL5 transcripts. The results showed that inoculation with SCN had no significant effect on the infection rate of AM fungi. However, the inoculation rate of SCN-inoculated SCN inoculated with AM fungi was significantly lower than that of SCN-inoculated SCN alone. In addition, the activities of chitinase and PAL in soybean roots inoculated with SCN were significantly increased after inoculation of AM fungi, and the peak of activity appeared on the 3rd day after inoculation of nematodes. It is worth noting that the peaks of Chib1 and PAL5 transcripts of two genes in soybean root inoculated with AM fungi after SC AM inoculation were also appeared on the 3rd day after SCN inoculation, that is, the infection rate of AM fungi increased rapidly and the SCN infection rate was rapid Declining period. Therefore, the expression of Chib1 and PAL5 genes may be a manifestation of AM fungi-induced defense against soybean cyst nematode disease. Therefore, it is speculated that Chib1 and PAL5 are directly involved in the defensive response induced by AM fungi to soybean cyst nematode.