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逆转录聚合酶链反应(RTPCR)法为目前运用最广泛的癌转移基因诊断方法,运用本法可检测恶性肿瘤患者淋巴结、外周血或骨髓中组织特异性基因的表达,从而诊断用传统的方法不能检测到的微小转移(micrometastasis)。人们在运用本法时常同时设立一个内对照标准以
Reverse transcription polymerase chain reaction (RTPCR) is the most widely used cancer metastasis gene diagnosis method. This method can detect the expression of tissue-specific genes in lymph nodes, peripheral blood, or bone marrow of patients with malignant tumors, so as to use traditional methods for diagnosis. Micrometastasis cannot be detected. People often use this law to establish an internal control standard at the same time.