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研究了阿片类药物在豚鼠离体回肠中的交互依赖性和交互耐受性．豚鼠回肠分别与羟甲芬太尼（ＯＭＦ１ｎｍｏｌ·Ｌ－１），Ｄ－Ａｌａ２，Ｄ－Ｌｅｕ５－脑啡肽（ＤＡＤＬＥ，０．３μｍｏｌ·Ｌ－１）或Ｕ５０４８８Ｈ（５０ｎｍｏｌ·Ｌ－１）在３７℃温育４ｈ，它们相互及自身均能抑制纳洛酮（０．１μｍｏｌ·Ｌ－１）或Ｍｒ２２６６（０．１μｍｏｌ·Ｌ－１）引起的戒断性收缩．它们也能自身预先阻断戒断性收缩的产生．Ｕ５０４８８Ｈ能预先阻断ＯＭＦ，ＤＡＤＬＥ温育后纳洛酮所产生的戒断性收缩，但后两者不能预先阻断Ｕ５０４８８Ｈ温育后由Ｍｒ２２６６所产生的戒断性收缩，并且ＯＭＦ和ＤＡＤＬＥ之间也不能相互阻断．豚鼠回肠分别与ＯＭＦ（１ｎｍｏｌ·Ｌ－１），ＤＡＤＬＥ（０．３μｍｏｌ·Ｌ－１）在３７℃温育２ｈ后，对去甲吗啡（ＮＭ），ＯＭＦ，ＤＡＤＬＥ的敏感性明显下降，浓度反应曲线右移，但对Ｕ５０４８８Ｈ不产生耐受．在Ｕ５０４８８Ｈ（１０ｎｍｏｌ·Ｌ－１）中温育１ｈ的回肠，对Ｕ５０４８８Ｈ的敏感性下降，浓度反应曲线右移，但对ＯＭＦ，ＮＭ，ＤＡＤＬＥ的敏感性不变．这些结果表明豚鼠回肠中μ，κ阿片受体是分离的．
The interactive dependence and interactive tolerance of opioids in isolated ileum of guinea pigs were studied. The ileum of guinea pigs were significantly inhibited by OMF1nmol·L-1, D-Ala2, D-Leu5-enkephalin (0.3μmol·L-1) or U50488H (50nmol·L- ℃ incubated for 4h, they can inhibit each other and their own naloxone (0.1μmol·L-1) or Mr2266 (0.1μmol·L-1) induced withdrawal contractility. They can also block the withdrawal of withdrawal in advance. U50488H can pre-block the abstinence produced by naloxone after OMF and DADLE incubation, but the latter two can not pre-block withdrawal-induced contractions caused by Mr2266 after U50488H incubation and the difference between OMF and DADLE Neither can we block each other. The sensitivities of NMD, OMF and DADLE to guinea pig ileum were significantly decreased with OMF (1nmol·L-1) and DADLE (0.3μmol·L-1) respectively at 37 ℃ for 2h, The curve moves to the right, but no tolerance to U50488H. In U50488H (10nmol·L-1) incubated for 1h in the ileum, the sensitivity of U50488H decreased, the concentration response curve shifted to the right, but the sensitivity of OMF, NM, DADLE remained unchanged. These results indicate that the μ, κ opioid receptors are isolated in the ileum of guinea pigs.