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稻纵卷叶螟颗粒体病毒(Cnaphalocrocis medinalis granulovirus,CnmeGV)于1979年首次从我国水稻主要害虫稻纵卷叶螟Cnaphalocrocis medinalis Guenée幼虫中分离,能够在宿主种群内传播,具备发展为生物杀虫制剂的潜力。本研究利用从田间采集的稻纵卷叶螟幼虫,在实验室成功增殖了CnmeGV。编码杆状病毒RNA聚合酶两个亚基的基因lef-8、lef-9和包涵体基因granulin在杆状病毒系统演化过程中非常保守。利用PCR扩增得到CnmeGV的上述三个基因,并将其核苷酸序列融合后与GenBank中相应病毒序列进行系统发育分析,UPGMA演化树分析结果表明CnmeGV是未被GenBank收录的病毒。流行病学初步调查结果表明,仅在采集自广东省江门市恩平县的稻纵卷叶螟体内发现了CnmeGV,而其他采集地点,包括广州市岑村、惠州市万芦村、惠州市新岗村和湖北省潜江市均未发现。江门市恩平县是CnmeGV的首次发现地,时隔30多年,仍然存在因感染CnmeGV而死亡的稻纵卷叶螟幼虫,表明该病毒具有田间控制目标害虫的持效性能。
Cnaphalocrocis medinalis granulovirus (CnmeGV) was first isolated from larva of Cnaphalocrocis medinalis Guenée, a major rice pest in China, in 1979 and could be propagated in the host population with the potential to develop into a bio-insecticide potential. In this study, CnmeGV was successfully propagated in laboratory using larvae of C. medinalis collected from the field. The lef-8, lef-9, and granulin genes encoding both subunits of the baculovirus RNA polymerase are very conserved during the baculovirus evolution. The above three genes of CnmeGV were amplified by PCR, and their nucleotide sequences were fused and phylogenetically analyzed with the corresponding virus sequences in GenBank. The results of UPGMA analysis showed that CnmeGV was not included in GenBank. The preliminary epidemiological findings showed that CnmeGV was found only in rice leafrollers collected from Enping County, Jiangmen City, Guangdong Province. Other sampling sites included Cencun in Guangzhou, Wanlucun in Huizhou, Xingang in Huizhou Qianjiang City, Hubei Province and the city were not found. Enping County, Jiangmen City, was the first discovery of CnmeGV. After more than 30 years, there was still the larvae of Cnaphalocrocis medinalis died of CnmeGV infection, indicating that the virus has the field-controllable efficiency of target pests.