论文部分内容阅读
目的:探讨卡托普利与贝那普利对糖尿病大鼠心肌的保护作用。方法:雄性SD大鼠60只随机分为空白对照组(n=15)和糖尿病组(n=45)。糖尿病组用链脲佐菌素(STZ,50 mg/kg)ip建模后,再随机分为糖尿病对照组、卡托普利50 mg/(kg.d)治疗组和贝那普利10 mg/(kg.d)治疗组。灌胃治疗9周后,处死全部大鼠,取心肌组织,用SP免疫组化染色法检测Ⅰ,Ⅲ型胶原的含量及Fas/FasL蛋白的表达率。电镜下观察心肌纤维及胶原的变化。结果:与空白对照组相比,糖尿病对照组及两个治疗组的心脏指数明显增大(P<0.05);Fas蛋白的表达率及Ⅰ,Ⅲ型胶原的表达增加(P<0.05);FasL蛋白的表达率差异不显著。卡托普利和贝那普利治疗组,上述指标较糖尿病对照组有显著改善;但与贝那普利治疗组相比,卡托普利组心脏指数及Ⅰ,Ⅲ型胶原的含量改善效果较差(P<0.05)。结论:糖尿病大鼠心肌中,有心肌细胞调亡和心肌间质重构发生。卡托普利与贝那普利可通过降低Fas和Ⅰ,Ⅲ型胶原的表达,明显改善心脏功能,但二者的效果稍有差别。
Objective: To investigate the protective effects of captopril and benazepril on myocardium in diabetic rats. Methods: Sixty male SD rats were randomly divided into blank control group (n = 15) and diabetic group (n = 45). Diabetic patients were treated with streptozotocin (STZ, 50 mg / kg) ip and then randomly divided into diabetes control group, captopril 50 mg / (kg.d) treatment group and benazepril 10 mg /(kg.d) treatment group. After gavage for 9 weeks, all the rats were sacrificed and the myocardial tissues were taken out. The content of type I and type III collagen and the expression of Fas / FasL protein were detected by SP immunohistochemistry. Electron microscope observation of myocardial fibrosis and collagen changes. Results: Compared with the blank control group, the cardiac index of diabetic control group and the two treatment groups were significantly increased (P <0.05); the expression of Fas protein and the expression of type I and type III collagen increased (P <0.05) The difference of protein expression rate was not significant. Captopril and benazepril treatment group, the above indicators were significantly improved compared with the diabetic control group; but compared with benazepril treatment group, captopril group heart index and type Ⅰ, Ⅲ collagen content than the improvement effect Poor (P <0.05). Conclusion: Cardiac myocytes in diabetic rats have cardiomyocyte apoptosis and myocardial interstitial remodeling. Captopril and benazepril can significantly improve cardiac function by reducing the expression of Fas and collagen type I and type III, but the effect is slightly different.