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目的 探讨婴幼儿血清肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)水平改变及IL-10/TNF-α比值与心力衰竭(CHF)严重程度之间的关系。方法 采用双抗体夹心酶联免疫吸附试验（ELISA）测定血清 TNF-α、IL-10水平。结果 重度CHF患儿血清TNF-α水平(861±223 ng/L)明显高于轻、中度CHF组(574±197 ng/L)及对照组(209±42 ng/L)。血清IL-10水平各组间无显著性差异(P＞ 0.05)，IL-10/TNF-α与婴幼儿CHF评分呈负相关(r=-0.36 P＜0.05)。结论 TNF-α、IL-10作为CHF发病机制的潜在介质，在婴幼儿CHF的发生和发展中起着重要作用。
Objective To investigate the changes of serum levels of tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and IL-10 / TNF-α in sera of infants and young children and the severity of heart failure. Methods Serum levels of TNF-α and IL-10 were measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results Serum levels of TNF-α in severe CHF patients (861 ± 223 ng / L) were significantly higher than those in mild and moderate CHF patients (574 ± 197 ng / L) and controls (209 ± 42 ng / L). Serum IL-10 levels were not significantly different among the groups (P> 0.05). There was a negative correlation between IL-10 / TNF-α and infant CHF (r = -0.36 P <0.05). Conclusion TNF-α and IL-10, as potential mediators of CHF pathogenesis, play an important role in the pathogenesis and development of CHF in infants.