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序言种间、属间的远缘杂交,在受精早期发生胚珠退化,往往得不到杂交种。在这种情况下,考虑到应用胚珠培养法做为获得杂交种的方法。烟草属的种间杂交,烟草与黄花烟草(Njcociana ruscica)杂交,是难以获得杂种的组合之一。以后者做父本时,花粉管伸长被限制在花柱内,连受精都不能完成。以前者做父本,虽然受精,但在胚发育的极早期就发生胚珠退化,只是极少获得结实种子而已。烟草属的许多种中,只有上述两个种才是栽培种,它们杂交具有实际育种意义。因此,本试验将胚珠培养法应用于这两个种的种间杂交,以探讨这种方法的效果。
Preamble inter-species, intercultural distant hybridization, in the fertilization early ovule degeneration, often do not get hybrids. In this case, it is considered that the ovule culture method is used as a method for obtaining hybrids. Interspecific hybridization of Nicotiana species, crossing of N. tabacum with Njcociana ruscica, is one of the most difficult combinations to obtain hybrids. The latter father, the pollen tube elongation is limited to the style, even fertilization can not be completed. The former father, although fertilized, but very early in the embryo development of ovule degradation, but rarely get seed only. Of the many species of Nicotiana, only the two species are cultivars, and their hybridization is of practical breeding significance. Therefore, in this study, ovule culture method was applied to the interspecific hybridization between the two species to explore the effect of this method.