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目的:探讨核糖核苷酸还原酶M2(RRM2)在人胆管癌组织及胆管癌细胞系中的表达、分布以及生物学意义。方法:采用免疫组织化学技术(SP法)检测RRM2基因和蛋白在40例胆管癌标本、40例癌旁胆管组织以及10例正常胆管组织标本中的表达;同时采用RT-PCR法及Western blot法分别检测了RRM2基因mRNA及其蛋白在人胆管癌细胞系QBC939以及人正常胆管上皮细胞系HIBEC中的表达。结果:RRM2基因蛋白在胆管癌组织中表达阳性率为72.5%,而在癌旁胆管组织和正常胆管组织中未能检测到RRM2表达。在人胆管癌细胞系QBC939中,RRM2的mRNA及蛋白均特异性表达,而在正常人胆管上皮细胞系HIBEC中未见RRM2的表达。结论:RRM2在人类胆管癌组织和胆管癌细胞系中选择性高表达,可能与胆管癌的发生、发展密切相关。
Objective: To investigate the expression, distribution and biological significance of ribonucleotide reductase M2 (RRM2) in human cholangiocarcinoma and cholangiocarcinoma cell lines. Methods: The expression of RRM2 gene and protein was detected by immunohistochemistry (SP method) in 40 cholangiocarcinoma specimens, 40 paracarcinoma adjacent bile duct tissues and 10 normal bile duct tissues. RT-PCR and Western blot The mRNA and protein expression of RRM2 gene were detected in human cholangiocarcinoma cell line QBC939 and human normal bile duct epithelial cell line HIBEC respectively. Results: The positive expression rate of RRM2 protein in cholangiocarcinoma was 72.5%, while the expression of RRM2 in paracancerous bile duct tissue and normal bile duct tissue was not detected. In human cholangiocarcinoma cell line QBC939, mRNA and protein of RRM2 were all specifically expressed, while no expression of RRM2 was found in HIBEC of normal human cholangiocarcinoma cell line. Conclusion: RRM2 is highly expressed in human cholangiocarcinoma and cholangiocarcinoma cell lines, which may be closely related to the occurrence and development of cholangiocarcinoma.