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目的:考察新疆树莓、黑果悬钩子和天山茶藨不同萃取部位的抗氧化活性及其对蛋白酪氨酸磷酸酶1B(PTP1B)的抑制作用,筛选具有抗氧化及降血糖活性的有效部位。方法:采用甲醇提取、分步萃取的方法分别制备了不同的极性部位。测定了3种植物不同部位的总黄酮含量,果实提取物经AB-8型大孔树脂纯化后制备了果实花青素部位;利用2,2’-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐法,1,1-二苯基-2-三硝基苯肼法及PTP1B抑制活性实验比较了不同部位的相关活性,并分析了黄酮含量和活性的相关性。结果:经AB-8型大孔树脂纯化后,树莓果实花青素含量由(12.72±0.14)mg·g-1提高到(238.13±1.18)mg·g-1,天山茶藨果实的花青素含量由(35.36±0.45)mg·g-1提高到(548.05±1.11)mg·g-1,随着花青素含量的升高,其抗氧化活性和PTP1B抑制活性也显著升高。黑果悬钩子茎乙酸乙酯部位总黄酮含量最高,为(658.65±11.43)mg·g-1;黑果悬钩子茎水部位抗氧化活性最高,ABTS和DPPH自由基清除能力分别为(4.53±0.07),(2.82±0.09)mmol Trolox·g-1当量;黑果悬钩子茎水部位对PTP1B的抑制率最高,半数抑制浓度为(0.08±0.01)mg·L-1。结论:这3种植物不同萃取部位均具有良好的抗氧化活性和PTP1B抑制作用,黑果悬钩子茎水部位为主要的抗氧化活性和PTP1B抑制活性的有效部位,树莓和天山茶藨果实中花青素是主要的活性成分,天山茶藨茎叶中黄酮是主要的抗氧化活性成分。
OBJECTIVE: To investigate the anti-oxidative activities and inhibitory effects of PTP1B on the different extraction sites of raspberry, raspberry, and Tianshan acanthopanax in Xinjiang, and to screen the effective sites of anti-oxidation and hypoglycemic activity . Methods: Different polar sites were prepared by methanol extraction and fractional extraction respectively. The content of total flavonoids in different parts of the three plants was determined. The anthocyanin parts of fruit extracts were prepared by AB-8 macroporous resin. Thiazole-6-sulfonic acid) diammonium salt method, 1,1-diphenyl-2-trinitrophenylhydrazine method and PTP1B inhibitory activity were compared in different parts of the activity, and analysis of flavonoid content and activity Correlation. Results: The content of anthocyanin in raspberry fruits increased from (12.72 ± 0.14) mg · g-1 to (238.13 ± 1.18) mg · g-1 after purification by AB-8 macroporous resin. The content of chlorophyll increased from (35.36 ± 0.45) mg · g-1 to (548.05 ± 1.11) mg · g-1. With the increase of anthocyanin content, the antioxidant activity and PTP1B inhibitory activity increased significantly. The highest content of total flavonoids was in the ethyl acetate fraction of A. przewalskii (658.65 ± 11.43) mg · g-1. The highest antioxidant activity in the stems and leaves of A. pseudosinus was (4.53 ± 0.07) and (2.82 ± 0.09) mmol Trolox · g-1 equivalents, respectively. The highest inhibitory concentration of PTP1B was found in the stem water of A. japonica. The half inhibitory concentration was 0.08 ± 0.01 mg · L-1. CONCLUSION: All three plants have good antioxidant activity and PTP1B inhibitory activity in different extraction parts. The stems and leaves of A. pseudogeus are the main active sites for antioxidant activity and PTP1B inhibitory activity. Anthocyanin is the main active ingredient, Tianshan tea stems and leaves of flavonoids is the main antioxidant activity.