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研究了郁金香(Tulipa gesneriana)中黄酮葡糖苷酸成分的血管保护作用。将郁金香干燥花被(500g)的甲醇提取液浓缩,并用氯仿和水萃取,水溶性成分经聚酰胺SC-6柱层析分离,依次以水、甲醇及含0.1％氨的甲醇洗脱。洗脱液重新柱层析,以水-正丁醇-乙酸(18:1:1)洗脱,得到提取物0.8g。经TLC检查,其中含有6种山柰酚和槲皮素的3-O-二糖苷-7-O-葡糖苷酸化合物。雄性家兔(平均体重2kg)分成三组,每组12只,均腹腔注射给药,一组给0.9％NaCl溶液(1mg/kg)作阴性对照;一组给郁金香提取物
The vascular protection of flavonoid glucuronide in tulipa (Tulipa gesneriana) was studied. The methanol extract of dried tulip (500 g) was concentrated and extracted with chloroform and water. The water-soluble fraction was separated by column chromatography on a polyamide SC-6 and eluted with water, methanol, and methanol containing 0.1% ammonia. The eluate was rechromatographed and eluted with water-n-butanol-acetic acid (18:1:1) to obtain 0.8 g of extract. Upon examination by TLC, it contained 6 kinds of 3-O-diglycoside-7-O-glucuronide compounds of kaempferol and quercetin. Male rabbits (average body weight 2 kg) were divided into three groups of 12 in each group and were given intraperitoneally. One group was given 0.9% NaCl solution (1 mg/kg) as a negative control; one group was given a tulip extract.