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目的了解和评估四川省区县疾控疟原虫镜检人员的检测技术、血片制作和染色水平,提高镜检质量,为顺利实施《四川省消除疟疾行动计划(2010-2012年)》目标提供科学依据。方法以全省所有区县为单位收集、登记2010年至2013年5月30日的阳性血片,由省疾控具有相当经验的镜检技术人员进行逐片复核评分。结果根据网络直报系统及防治工作年报表,预估计应收总疟原虫血片数(网络直报血检阳性数)623张,实际上交阳性血片数224张,实际收片率为35.96%;复核224张血片,复检符合率62.95%;其中疑似血片(初检为阴性血片)和初检为阳性血片复检符合率分别为78.79%、60.21%;血片制作和染色优中等血片分别占75.89%、78.59%。结论四川省基层疟疾防治疟原虫镜检人员血片保存率、镜检符合率不高,存在漏检、虫种错检、假阳性及制作染色质量不佳等问题。
Objectives To understand and assess the detection techniques, blood film production and staining levels of Plasmodium falciparum malaria parasite in Sichuan Province and to improve the quality of microscopic examination for the smooth implementation of the “Sichuan Province Action Plan for Malaria Elimination (2010-2012)” Scientific basis. Methods All the districts and counties in the province as a unit to collect and register the positive blood film from 2010 to May 30, 2013, the provincial CDC has considerable experience in microscopic examination technicians on a one-by-one review score. Results According to the network direct reporting system and prevention and cure work annual report, it is estimated that 623 pieces of the total number of blood samples of paralysis should be collected (network direct blood test positive number), in fact, the number of positive blood transfusions is 224 and the actual closing rate is 35.96 %; 224 blood films were reviewed, the coincidence rate of re-examination was 62.95%; the coincidence rates of the suspected blood film (initial blood test) and the first blood test were 78.79% and 60.21% respectively; Dyeing excellent middle class blood film accounted for 75.89%, 78.59%. Conclusion The malaria parasite control rate in Sichuan province is very low. The coincidence rate of microscopic examination is not high. There are some problems such as missed inspection, false positives, poor quality of dyeing and so on.