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目的评价丙烯酰胺(acrylamide,AA)对睾丸细胞DNA损伤及精子核成熟率的影响,为研究AA生殖系统损伤机制提供实验室数据。方法以20、40和60 mg/kg AA经口灌胃染毒雄性小鼠,并设对照组,连续给药5 d,首次给药后14 d时,用彗星实验和吖啶橙荧光染色法检测睾丸细胞DNA损伤和小鼠精子核成熟率。结果彗星实验结果显示:60 mg/kg AA组中各监测指标与对照组比较,差异均有统计学意义(P<0.01),且随AA剂量的增加,各指标均明显增高,有显著的剂量-效应关系。吖啶橙荧光染色法结果显示:40和60 mg/kg剂量组小鼠精子核成熟率与对照组比较,差异有统计学意义(P<0.01),且随着染毒剂量的增加,精子核成熟率逐渐下降。结论 AA对小鼠睾丸细胞DNA有持续损伤作用,并与染毒剂量明显相关。AA破坏精子核DNA的完整性,导致精子核成熟率降低,以致于精子受精能力降低。
Objective To evaluate the effect of acrylamide (AA) on DNA damage and sperm nuclear maturation in testis cells and provide laboratory data for studying the mechanism of AA reproductive system injury. Methods Male mice were intragastrically administrated with 20, 40 and 60 mg / kg AA, respectively. The rats in the control group were dosed continuously for 5 days. At 14 days after the first administration, the mice were treated with comet assay and acridine orange fluorescence staining Detection of DNA damage in testicular cells and mouse sperm maturation rate. Results The results of comet assay showed that there was significant difference between the control group and the 60 mg / kg AA group (P <0.01). With the increase of AA dose, all the indexes were significantly increased, with significant dose - the relationship between effects. Acridine orange fluorescence staining showed that there was a significant difference in sperm maturation rate between the 40 and 60 mg / kg groups compared with the control group (P <0.01). With the increase of the dose of sperm nucleus The maturity rate gradually decreased. Conclusion AA has a sustained damage on the DNA of testicular cells in mice and is significantly related to the dose of exposure. AA destroys the integrity of sperm DNA, resulting in reduced sperm nuclear maturation, resulting in reduced sperm fertility.