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目的探讨重组人促红细胞生成素(rhEPO)对宫内感染致早产新生大鼠脑损伤后的神经保护作用。方法妊娠18 d孕鼠随机分为对照组、感染组和干预组,对感染组及干预组分别给予LPS剂量0.45 mg.kg1d腹腔内注射,连用2d,对照组按同样方法注射生理盐水,24 h后处死大鼠,取胎盘组织行HE染色观察其病理变化,余孕鼠继续怀孕至自然分娩,干预组新生鼠d 1腹腔注射重组人促红细胞生成素5 000 IU.kg1d,连续注射3 d,部分待新生鼠6日龄大取其大脑,免疫组织化学法检测其大脑髓磷脂碱性蛋白(MBP)宫内感染情况下及干预后的不同表达变化,余新生早产鼠饲养至42 d大小行迷宫试验。结果与对照组比较,感染及干预组孕鼠胎盘组织HE染色后镜下观察均可见明显的炎性白细胞浸润,血管充血等病理改变,对照组、感染组、干预组MAP的表达变化分别为:对照组(127.043.891)、感染组(149.683.189)和干预组(131.123.467),感染组MAP的表达低于对照组及干预组,干预组及对照组无明显差别;感染组大鼠Y型迷宫试验达标所需的训练次数较对照组及干预组明显增多,分别为:对照组(56.75.2)、感染组(145.89.6)和干预组(58.47.7),记忆保持中正确反应次数明显低于对照组及干预组,分别为:对照组(0.920.06)、感染组(0.520.09)和干预组(0.900.09),干预组及对照组无明显差异。结论宫内感染可使早产新生大鼠脑组织MBP的表达减少,而重组人促红细胞生成素可促进少突胶质细胞生成,影响早产新生大鼠髓鞘的形成并提高认知,改善新生大鼠的学习记忆功能,影响其远期智能发育。
Objective To investigate the neuroprotective effect of recombinant human erythropoietin (rhEPO) on brain injury induced by intrauterine infection in premature newborn rats. Methods Pregnant mice were randomly divided into control group, infection group and intervention group on the 18th day of pregnancy. The infected group and the intervention group were given intraperitoneal injection of 0.45 mg.kg LPS for 2 days respectively. The control group received normal saline 24 h After the rats were sacrificed, the placenta were removed and their pathological changes were observed by HE staining. The remaining pregnant rats continued to be pregnant until spontaneous delivery. The neonatal rats in the intervention group were intraperitoneally injected with 5000 IU.kg of recombinant human erythropoietin 5000 for 1 d, Some newborn rats were taken at 6 days old to take their brains, immunohistochemistry was used to detect changes in their intrauterine infection of myelin basic protein (MBP) and their expression after intervention. Yu Xinsheng preterm rats were housed for 42 days Maze test. Results Compared with the control group, the placental tissue of infected and intervention group showed obvious inflammatory leukocyte infiltration and vascular congestion after HE staining. The changes of MAP in the control group, infection group and intervention group were as follows: In the control group (127.043.891), the infection group (149.683.189) and the intervention group (131.123.467), the expression of MAP in the infected group was lower than that in the control group and the intervention group. There was no significant difference between the intervention group and the control group. Y-shaped maze test required training times than the control group and the intervention group was significantly increased, respectively: control group (56.75.2), infection group (145.89.6) and intervention group (58.47.7), memory retention is correct The number of reaction was significantly lower than that of the control group and intervention group, respectively: control group (0.920.06), infection group (0.520.09) and intervention group (0.900.09), intervention group and control group had no significant difference. Conclusion Intrauterine infection can reduce the expression of MBP in the brain tissue of premature neonatal rats, and recombinant human erythropoietin can promote the formation of oligodendrocytes, affect the formation of myelin in premature neonatal rats and improve their cognition, Rat learning and memory function, affecting its long-term intelligent development.