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目的:探索监测动脉血压的清醒大鼠重症中暑模型的构建方法。方法:随机将40只雄性大鼠分为清醒中暑组、麻醉中暑组、清醒对照组和麻醉对照组。其中,清醒中暑组的大鼠在腹腔注射麻醉情况下完成右侧颈总动脉置管并固定,待其清醒后再置于高温高湿环境接受热打击,直至其体温超过42℃及血压下降;麻醉中暑组在动脉置管后保持大鼠在麻醉状态接受热打击;清醒、麻醉对照组则置于常温常湿环境中。比较各组生命体征、热负荷量、体重丢失、生存率指标的变化。结果:较麻醉中暑组而言,清醒对照组具有显著增加的热负荷量及体重丢失,其生命体征变化更平缓、生存率也更高。结论:监测动脉血压的清醒大鼠模型更加符合重症中暑致病过程中的病理生理学特点,更能满足后续动物实验的需要。
OBJECTIVE: To explore a method for constructing a rat model of severe heat stroke that monitors arterial pressure. Methods: Forty male rats were randomly divided into awake heat stroke group, anesthetized heat stroke group, awake control group and anesthetized control group. Among them, the rats in awake heat stroke group completed the right common carotid artery cannulation and fixation under the condition of intraperitoneal injection of anesthesia. After awake, they were placed in a hot and humid environment for heat shock until their body temperature exceeded 42 ℃ and their blood pressure dropped. After anesthesia heat stroke group, rats were kept in anesthetized state and received thermal shock after arterial catheterization. The conscious and anesthetized control group was placed in normal temperature and humidity environment. The changes of vital signs, heat load, weight loss, and survival rate were compared between groups. Results: Compared with the anesthetized sunstroke group, the awake control group had a significantly increased heat load and weight loss with a more gradual change of vital signs and a higher survival rate. Conclusion: The rat model of arterial blood pressure monitoring is more in line with the pathophysiology of severe heat stroke pathogenesis, which can better meet the needs of follow-up animal experiments.