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背景:幽门螺杆菌（H.pylori）疫苗是近年研究的热点。H.pylori尿素酶B亚单位（UreB）是理想的候选抗原。目的:获得纯化的重组H.pylori UreB（rUreB）,为进一步的H.pylori疫苗制备打下基础。方法:采用金属螯合亲和层析法（MCAC）在变性条件下纯化rUreB,SDS-聚丙烯酰胺凝胶电泳（PAGE）和免疫印迹法鉴定纯化产物的分子量、纯度和抗原性。结果:纯化产物的分子量与预计分子量相符,具有良好的特异性UreB免疫原性,且一步即可达到90％以上的纯度。结论:MCAC是一种简单、高效的rUreB纯化方法。
Background: Helicobacter pylori (H. pylori) vaccine is a hot topic in recent years. H. pylori urease B subunit (UreB) is the ideal candidate antigen. OBJECTIVE: To obtain purified recombinant H.pylori UreB (rUreB), which laid the foundation for the further preparation of H.pylori vaccine. Methods: The molecular weight, purity and antigenicity of the purified product were identified by the purification of rUreB under denaturing conditions by metal chelate affinity chromatography (MCAC). SDS-polyacrylamide gel electrophoresis (PAGE) and Western blotting were used to identify the molecular weight, purity and antigenicity. Results: The purified product had a molecular weight consistent with the predicted molecular weight, had good specificity for UreB immunogenicity, and achieved a purity of 90% in one step. Conclusion: MCAC is a simple and efficient rUreB purification method.