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[目的]从枫香(Liquidambar formosana Hance)根际土壤中分离和鉴定有效解磷菌(PSB),并研究其在溶磷方面的应用.[方法]采用形态学及生理生化特征、微生物鉴定系统(MIDI)、BIOLOG细菌鉴定和16SrDNA序列分析等方法鉴定解磷菌.应用离子发射光谱法测定各解磷菌菌株(JX7、JX18、JX21、WHP)处理下枫香叶片大量及微量元素的含量,鉴定PSB菌株对植物生长的影响.[结果]解磷细菌菌株JX7鉴定为争论产碱菌(Variovorax para?doxus),JX18鉴定为蜡状芽孢杆菌(Bacillus cereus),WH10鉴定为蕈状芽孢杆菌(Bacillus mycoides),JX21鉴定为蜡状芽孢杆菌(Bacillus cereus).菌株JX21的可溶性磷酸盐含量最高,为(9.35±0.89)mg/L,其次是JX7[(6.63±0.09)mg/L],WH10[(6.16±0.12)mg/L]和JX18[(5.32±0.07)mg/L].以上解磷菌在培养72 h后,其溶磷量均达到最大值.筛选自枫香根际的解磷菌菌株(JX7、WH10、JX18、JX21)接种枫香幼苗比对照显著提高了枫香的苗高和地径(P<0.05).在室温条件下接种解磷菌显著提高枫香叶片内的P、Ca、Mg、Zn的含量(P<0.05).[结论]筛选解磷菌均显示出良好的溶磷特性,并具有作为生物肥料的潜力.可进一步评估所筛选菌株的特性,开发其商业应用.“,”[Objective]Isolate and identify efficient phosphate solubilizing bacteria(PSB)from Liquidambar formosanaHance rhizosphere soil and investigate the application in phosphorus release.[Method]the PSB strains were graded based on morphological and physiological?biochemical characteristics,microbial identification system(MIDI),BIO?LOG analysis and 16S rRNA identification methods. The effect of these PSB strains(JX7,JX18,JX21 and WH10)on plant growth was evaluated by determining the minor and major mineral contents of L. formosana leaves using inductive?ly?coupled plasma atomic emission spectrometry.[Result]Isolates JX7,JX18,WH10 and JX21 were closely related to Variovorax paradoxus,Bacillus cereus,B. mycoides and B.cereus,respectively. The JX21 showed the highest content of soluble phosphate was(9.35 ± 0.89)mg/L,followed by JX7[(6.63±0.09)mg/L],WH10[(6.16 ± 0.12 )mg/L]andJX18[(5.32 ± 0.07 )mg/L],all the maximum phosphate was fermented after 72 h of incubation. Phosphate?solubilizing bacteria(JX7,WH10,JX18 and JX21)selected from L. formosana rhizosphere showed a significantly improvement in shoot height and ground diameter of L. formosana(P<0.05). As well as,these selected strains promoted L. formosana in taking up mineral elements such as phosphorus,magnesium,calcium and zinc under greenhouse conditions.[Conclu?sion]The isolates showed good phosphate?solubilizing activity and had potential for use as biofertilizer agents. Further study is needed to evaluate beneficial traits to develop commercial applications.