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目的了解艾滋病病毒I型(HIV-1)Gag和Env蛋白特异性抗体依赖性细胞介导的细胞毒作用(ADCC)反应,与艾滋病(AIDS)疾病进展的相关性。方法采集58份未经抗病毒治疗的、感染时间1年以内的男男性行为人群(MSM)标本,利用HIV-1特异性肽库刺激和流式细胞染色技术进行ADCC检测。结果病毒载量与HIV-1Gag特异性CD+2IFN-γ+细胞占CD-3细胞的比例呈显著的正相关(r=0.2919,P=0.0339);而01_AE亚型感染者的病毒载量与HIV-1Env特异性CD+2CD107a+细胞占CD-3细胞的比例呈显著负相关(r=-0.3454,P=0.0454)。结论在HIV-1感染早期,感染者体内可以产生Gag蛋白特异性的ADCC反应,其中Env蛋白特异性的ADCC反应具有控制疾病进展的作用。
Objective To investigate the association of HIV-1 Gag and Env protein-specific antibody-dependent cell-mediated cytotoxicity (ADCC) responses with the progression of AIDS. Methods Fifty-eight MSM specimens without infection and within 1 year of infection were collected and ADCC was detected by HIV-1-specific peptide pool stimulation and flow cytometry. Results The viral load was positively correlated with the ratio of HIV-1 Gag-specific CD + 2IFN-γ + cells in CD-3 cells (r = 0.2919, P = 0.0339) There was a significant negative correlation between HIV-1 Env-specific CD + 2CD107a + cells and CD-3 cell ratio (r = -0.3454, P = 0.0454). Conclusion In the early stage of HIV-1 infection, Gag protein-specific ADCC responses can be produced in infected individuals. The Env protein-specific ADCC response plays a role in controlling disease progression.