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皮肤表皮细胞尤其是成年的表皮细胞的体外培养研究是具有临床实践和一般理论意义的,可是这方面的文献报导并不很多,而且在培养方法上也还存在一些问题。我们以成年兔耳皮肤和成人腿部皮肤为试验材料。将皮肤在剃尽毛之后,用肥皂水洗净,经麻醉(兔子用戊巴比妥钠经腹腔注射作全身麻醉,必要时辅以乙醚;人体则用盐酸普洛卡因在皮下注射作局部麻醉)后以70%酒精和乙醚交替(3—4次)揩擦皮肤,可达彻底消毒(在冤耳缘和耳壳内侧涂以火棉胶液,对防止污染是有帮助的)。然后移入无菌手术室,加盖消毒巾,再经一次最后的酒精消
The study of in vitro culture of epidermal cells, especially adult epidermal cells is of clinical practice and general theoretical significance. However, there are not many reports in this field and there are still some problems in culture methods. We use adult rabbit ear skin and adult leg skin as the test material. After the skin is shaved, the skin is washed with soapy water and anesthetized (rabbits are anesthetized with sodium pentobarbital via intraperitoneal injection and supplemented with ether if necessary; the human body is subcutaneously injected locally with procaine hydrochloride Anesthetized) with 70% alcohol and ether alternating (3-4 times) 揩 rub the skin, up to complete disinfection (in the grievances and ear shell coated with fire cotton glue, to prevent contamination is helpful). Then moved to a sterile operating room, stamped with disinfectant towel, and then by a final alcohol consumption