Reservoirs tend to have enhanced methylmercury (MeHg) concentrations compared to natural lakes and rivers,and water level fluctuations can promote MeHg production.Until now,little research has been conducted on the effects of microorganisms in soils for the formation of MeHg during different drying and flooding altating conditions in the Three Gorges Reservoir (TGR).This study aimed to understand how water level fluctuations affect soil microbial composition and mercury concentrations,and if such microbial variations are related to Hg methylation.The results showed that MeHg concentrations and the ratios of MeHg to THg (MeHg％) in soils were higher in the seasonally drying and flooding altemating areas (DFAs,175-155 m) than those in the non-inundated (NIAs,＞175 m) and inundated areas (IAs,＜ 145 m).However,MeHg％ in all samples was less than 1％,indicating that the Hg methylation activity in the soils of the TGR was under a low level.454 high-throughput sequencing of 165 rRNA gene amplicons showed that soil bacterial abundance and diversity were relatively higher in DFA compared to those in NIA and IA,and microbial community composition varied in these three areas.At the family level,those groups in Deltaproteobacteria and Methanomicrobia that might have many Hg methylators were also showed a higher relative abundance in DFA,which might be the reason for the higher MeHg production in these areas.Overall,our results suggested that seasonally water level fluctuations can enhance the microbial abundance and diversity,as well as MeHg production in the TGR.