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目的了解江苏省徐州地区2009-2010年儿童手足口病流行的病原学特征。方法 2009年3-8月和2010年1-12月共采集徐州儿童医院门诊、住院及常规监测的511例疑似手足口病(HFMD)儿童患者的咽拭、肛拭标本,提取病毒RNA,用实时荧光定量PCR(real-time PCR)方法同时进行肠道病毒(EV),肠道病毒71型(EV71)和柯萨奇病毒A16(Cox A16)的特异性基因检测。结果 511例疑似手足口病患儿的标本中,总肠道病毒阳性288例,占56.36%;288个肠道病毒核酸阳性病例中,EV71核酸阳性202例,Cox A16核酸阳性55例,构成比分别为70.14%和19.10%。≤5岁儿童感染的肠道病毒有EV71型、Cox A16型及非EV71非Cox A16的其他肠道病毒感染,>5岁儿童则只有EV71感染病例。结论 2009-2010年徐州地区儿童手足口病患儿以1～3岁儿童为主,其主要病原是肠道病毒EV71型,其次是Cox A16型。
Objective To understand the etiological characteristics of HFMD in children from 2009 to 2010 in Xuzhou, Jiangsu Province. Methods From March to August in 2009 and January to December in 2010, a total of 511 children with suspected hand-foot-and-mouth disease (HFMD) in outpatient, inpatient and routine monitoring in Xuzhou Children’s Hospital were collected for pharyngeal swab and anal swabs, and viral RNA was extracted from Real-time PCR was used to detect the specific genes of enterovirus (EV), enterovirus 71 (EV71) and coxsackievirus A16 (Cox A16) at the same time. Results Among 511 cases of suspected HFMD, 288 cases were positive for total enterovirus, accounting for 56.36%. Of the 288 cases of enterovirus nucleic acid positive, 202 were positive for EV71 and 55 were positive for Cox A16, Respectively 70.14% and 19.10%. Other enteroviruses infected with children less than 5 years of age were infected with EV71, Cox A16 and non-EV71 non-Cox A16, while those with EV71 infection were> 5 years old. Conclusion From 2009 to 2010, children with HFMD in Xuzhou were mainly children aged 1-3 years. The main pathogen was EV71, followed by Cox A16.