According to the electric potential of oblique multi-needle electrodes (OMNE) in biological tissue, the discrete equations based on the indetermination linear current density were established by the boundary element integral equations (BEIE). The non-uniform distribution of the current flowing from multi-needle electrodes to conductive biological tissues was imaged by solving a set of linear equa- tions. Then, the electric field and potential generated by OMNE in biological tissues at any point may be determined through the boundary element method (BEM). The time of program running and stability of computing method are examined by an example. It demonstrates that the algorithm possesses a quick speed and the steady computed results. It means that this method has an important referenced sig- nificance for computing the field and the potential generated by OMNE in bio-tissue, which is a fast, effective and accurate computing method. According to the electric potential of oblique multi-needle electrodes (OMNE) in biological tissue, the discrete equations based on the indetermination linear current density were established by the boundary element integral equations (BEIE). The non-uniform distribution of the current flowing from Multi-needle electrodes to conductive biological tissues was imaged by solving a set of linear equa- tions. Then, the electric field and potential generated by OMNE in biological tissues at any point may be determined through the boundary element method (BEM). The time of program running and stability of computing methods are examined by an example. It demonstrates that the algorithm possesses a quick speed and the steady steady results. It means that this method has an important referenced sig- nificance for computing the field and the potential generated by OMNE in bio-tissue, which is a fast, effective and accurate computing method.