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采用砂培方法,在温室内研究了马尾松1年生苗木在不同锰浓度(0.005(对照)、1、5、10和15mmol·L-1)条件下的生理指标响应。结果表明:与对照相比,高锰浓度(≥10mmol·L-1)下马尾松的生物量和根系活力降低。高锰条件下,马尾松针叶中叶绿素a、叶绿素b、类胡萝卜素含量下降,表明锰对马尾松光合机构存在一定影响,同时也是导致生物量降低的原因之一。在高锰胁迫下,丙二醛(MDA)含量以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均发生显著变化且对锰毒起到一定的缓解作用,其中CAT和MDA对高锰胁迫的响应比较灵敏,MDA和2种酶的变化反映了马尾松对高锰胁迫的生理响应。
The sand culture method was used to study the physiological response of Pinus massoniana seedlings to different concentrations of manganese (0.005 (control), 1, 5, 10 and 15 mmol·L-1) in greenhouse. The results showed that compared with the control, the mass and root vigor of Pinus massoniana at high manganese concentration (≥10 mmol·L-1) decreased. The contents of chlorophyll a, chlorophyll b and carotenoid in the pine needles decreased with high manganese content, indicating that Mn had some influence on the photosynthetic mechanism of Pinus massoniana and was also one of the reasons leading to the decrease of biomass. Under high manganese stress, the content of malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and catalase (CAT) all changed significantly, which could alleviate the toxicity of manganese. CAT and MDA The response to high manganese stress was more sensitive. The changes of MDA and two enzymes reflected the physiological responses of P. massoniana to high Mn stress.