Differential thymosin β10 expression levels and actin filament organization in tumor cell lines with

来源 :Chinese Medical Journal | 被引量 : 0次 | 上传用户:fatcatgao
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Background To investigate the differential expression levels of thymosin β10 (Tβ10) and the corresponding changes of actin filament organization in human tumor cell lines with different metastatic potential.Methods Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the amount of Tβ10 mRNAs by Northern blot and for their peptide expression levels by immunohistochemistry. The filamentous actin (F-actin) was observed by staining of TRITC-phalloidin to detect changes in actin organization.Results In comparison with non-/weakly metastatic counterparts, Tβ10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non-/weakly metastatic cancer cell lines.Conclusion Tβ10 levels correla Background To investigate the differential expression levels of thymosin β10 (Tβ10) and the corresponding changes were 40 human tumor cell lines with different metastatic potential were analyzed for the amount of Tβ10 mRNAs by Northern blot and their peptide expression levels by immunohistochemistry were observed by staining of TRITC-phalloidin to detect changes in actin organization. Results In non- / weakly metastatic counterparts, Tβ10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. Staining of TRITC-phalloidin revealed less actin bundles, a fuzzy network of shorter filaments and some F-actin aggregates in the highly metastatic tumor cells. Meanwhile, the actin filaments were robust and orderly arranged in the non- / weakly metastatic cancer cell lines. Confluence T 10 levels correla
其他文献
赫伯特·吉拉尔代出生于德国。1963年移居英国,后居住在布里斯托尔(英国西部的港都)近30年。现为英国舒马赫基金会的主席、土壤协会的赞助人、西英格兰大学的教授。他是联合
我们可以用一个复合词汇来描述未来的世界--有限的生态容量.无论从投资者还是从人类自身的角度出发,地球有限的生态容量都是我们面临的最大挑战.我们已面临这一难题.然而,判
不久前,国家电监会建议将电价政策由招标定价制度改为固定电价制度.风电电价的转变,是否将对风电市场回暖发挥影响?
江河是人类最宝贵的淡水资源,灌溉了农田、哺育了人民。然而,世界上的大江大河正在陷入困境,水危机正在全球蔓延:在约旦河到达约旦之前,以色列人正用管道把它抽干;恒河流域连
Objective:The effect of tranexamic acid (TA) on patients receiving total knee arthroplasty (TKA) has been reported in many small clinical trials.But single tria
守望地球组织(EWI)成立于1971年,是一个国际性的非政府、非盈利性组织.它的主要工作是向那些环境保护研究组织输送志愿者,通过志愿者与科学工作者的共同合作,为那些关心环保
利用功率谱反演加次谐波的方法模拟产生了符合Kolmogorov统计规律的大气湍流畸变波前相位屏,重点分析了所生成的相位屏在旋转使用情形下的统计特性,为后续的相位屏旋转应用实
介绍了一种基于PIN光电二极管的紫外激光脉冲能量在线检测系统.首先,采用激光主光路外的检测光实现激光脉冲能量的无损在线响应;其次,采用高速高精度积分电路将响应光电流处
“木塑材料本身就是对循环经济最好的阐述,”中国资源综合利用协会木塑/复合材料专业委员会秘书长刘嘉对木塑行业的发展越来越充满信心.