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目的 :调查东莞地区TTV感染流行情况和基因分型。方法 :选以G1、G2、G3、G4、G5、G6 同源性极高的系列nt2160 -nt2196作包被探针 ,异源性大于20 %的序列作显色探针G1、G2、G3、G4、G5、G6,采用PCR微板核酸杂交ELISA技术对410例肝功能损害的肝炎患者血清进行TTV检测及TTV基因分型研究。结果 :检出66例TTV阳性患者 ,检出率为16 0 %。对66例TTV进行基因分型 ,G1 型47例 ,G2 型6例 ,G1、G2 混合型感染11例 ,G3、G4 各1例 ,尚未发现G5、G6 型。结论 :在东莞地区的肝炎病人中 ,TTV的感染率为16 0 % ;TTV基因型主要为G1 型 ,其次为G2 型 ,未发现G5、G6 型。
Objective: To investigate the prevalence and genotyping of TTV infection in Dongguan area. Methods: A series of probes nt2160-nt2196 with high homology of G1, G2, G3, G4, G5 and G6 were selected as probes, and the sequences with more than 20% G4, G5, G6. TPL detection and TTV genotyping were performed on 410 serum samples of hepatitis patients with hepatic impairment by ELISA using microplate nucleic acid hybridization. Results: 66 TTV positive patients were detected, the detection rate was 160%. TTV was genotyped in 66 TTV cases, including 47 cases of G1 type, 6 cases of G2 type, 11 cases of G1 and G2 mixed infection, 1 case of G3 and G4, and no G5 or G6. Conclusion: The prevalence of TTV in Dongguan patients was 16 0%. The genotypes of TTV were mainly G1, followed by G2, but not G5 and G6.