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在体外对淀粉的消化条件进行仿生模拟,对淀粉的消化产物检测条件进行优化,构建了淀粉体外消化模型。以环氧氯丙烷为交联剂,制备土豆交联淀粉,以构建的体外消化模型,对不同交联度淀粉的消化速度进行研究。结果显示,以恒温振荡培养箱保持37℃恒温,以pH6.9缓冲液维持恒定的pH,以20 r/min的转速振荡模拟肠的蠕动,以透析袋模拟肠,对淀粉的消化可很好的仿生模拟。采用硫酸-苯酚法,消化产物检测的最佳条件为∶波长485 nm,显色温度100℃,显色时间30 min,糖浓度在(0~80)μg/mL范围内线性关系良好,其回归方程为y=0.004 4x-0.005,R2=0.998 8,且样品溶液在2 h内显色稳定。土豆交联淀粉和交联前相比,消化性降低了13.7%~34.5%,且与交联度呈负相关,即交联度越高(沉降积越小),消化性就越低。
In vitro biomimetic simulation of starch digestion conditions, digestion products starch detection conditions were optimized to build a starch in vitro digestion model. Using epichlorohydrin as crosslinking agent, potato cross-linked starch was prepared. The in vitro digestion model was constructed to study the digestion rate of starch with different cross-linking degree. The results showed that constant temperature shaking incubator to maintain 37 ℃ to pH6.9 buffer to maintain a constant pH to 20 r / min speed simulation of simulated intestinal peristalsis dialysis bag simulated intestine, digestion of starch can be very good Bionic simulation. The optimum conditions for the detection of digestion products were as follows: the wavelength was 485 nm, the color temperature was 100 ℃, the color reaction time was 30 min, and the concentration of glucose was within the range of (0 ~ 80) μg / mL. The regression equation The equation is y = 0.004 4x-0.005, R2 = 0.998 8, and the sample solution is stable in color within 2 h. Digestibility of potato cross-linked starch decreased by 13.7% -34.5% compared with that before cross-linking, and negatively correlated with the degree of cross-linking, ie, the higher the degree of cross-linking (less sedimentation), the lower the digestibility.