从棉花转BT-CPTI双价抗虫基因后代中选育了核不育种质98-8A,采用表型观察、自交和测交手段,对其植株表型、花器、育性特征及其遗传规律进行了系统分析。花器官形态观察表明,不育株花柱长和花柱外露长度均明显高于同质系的正常可育株,花丝明显萎缩,不育株花药无花粉粒出现,而子房直径及花药数量没有明显差异;对不育株与可育株姊妹交后代及可育株自交后代遗传分析表明,该雄性不育性受隐性单基因控制;等位性检测显示,98-8A的不育基因与已知的473A(ms14/msc1)、1355A(ms2/msc2)、阆A(ms15/msc3)等3个不育基因为非等位基因。不育株与常规多品种杂交分析表明,98-8A的一般配合力与特殊配合力均较高,且衣分高、纤维品质较好,暗示有较好的应用前景。 The male sterile line 98-8A was bred from the progeny of the binary transgenic cotton BT-CPTI and its phenotype. The characteristics of its plant phenotype, flower arrangement, fertility and its inheritance The rules were systematically analyzed. Morphological observation of floral organs showed that the length of the flower style and the exposed length of the style were significantly higher than that of the normal fertile line of the homozygous line, the filament of the flower was obviously atrophied, the pollen grains of the anther of the sterile plant appeared, but the ovary diameter and the number of the anther were not obvious The genetic analysis of selfing offspring of sister and offspring of sterile and fertile plants showed that this male sterility was controlled by a recessive single gene. Allelism detection showed that the male sterile gene of 98-8A Three known sterility genes, ie, 473A (ms14 / msc1), 1355A (ms2 / msc2) and 阆A (ms15 / msc3) are non-alleles. Analysis of cross between sterile and conventional varieties showed that the general combining ability and the special combining ability of 98-8A were higher, and the higher parting percentage and better fiber quality suggested that 98-8A had a better application prospect.