论文部分内容阅读
目的 观察协同刺激分子4-1BB Ligand(4-1BBL)基因导入小鼠肝癌细胞Hrpal-6后在小鼠体内诱导的抗瘤效应。方法 应用逆转录病毒载体,将小鼠4-1BBL导入小鼠肝癌细胞Hepal-6细胞中,Histidinol(HisD)筛选后获高表达4-1BBL分子附性细胞克隆,经丝裂霉素C(MMC)处理制备成肿瘤细胞瘤苗TCV4-1BBL,观察其对下同动物模型的体内免疫保护作用和免疫治疗作用。结果(1)能对同系肝癌细胞产生完全的免疫保护作用,并能保持无瘤状志长期存活(100d以上)。(2)对早期(接种 7d)形成的肿瘤有强的治疗作用。(3)对晚期(接种14d)肿瘤,有明显的治疗作用,使大部分小鼠的肿瘤消退。结论4-1BBL修饰的肿瘤细胞疫苗能明显刺激增强带瘤宿主的抗瘤效应。
Objective To observe the anti-tumor effect induced by the co-stimulatory molecule 4-1BB Ligand (4-1BBL) gene in mouse hepatoma Hrpal-6 cells. Methods Mouse 4-1BBL was transfected into mouse Hepal-6 cells by retroviral vector. Clones with high expression of 4-1BBL were screened by Histidinol (HisD) and transfected with mitomycin C (MMC ) To prepare the tumor cell vaccine TCV4-1BBL, and observe its immunoprotective effect and immunotherapy on the same animal model in vivo. Results (1) It could completely protect the hepatoma cells and maintain the long-term survival (more than 100 days). (2) has a strong therapeutic effect on the tumors formed in the early stage (on day 7). (3) For advanced (14d) tumor, there is a significant therapeutic effect, and the tumor of most mice subsides. Conclusions The 4-1BBL modified tumor cell vaccine can significantly stimulate the antitumor effect of the tumor-bearing host.