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用外周血淋巴细胞短期培养法,对64头奶牛进行姐妹染色单体交换(SCE)频率的观察,结果表明,淋巴肉瘤牛、血检和MID双阳性牛、MID单阳性牛以及健康对照牛SCE/细胞值分别为9.36±0.349(S·E),6.60±0.495,4.89±0.379和3.59±0.328,各组之间有极显著差异(P<0.01)。同时,用等离子体源发射光谱分析法对牛毛Zn、Ni、Mn、Cu、Co、Pb、Mo、Cr、Ba、B10种元素进行测定,发现MID单阳性牛、血检和MID双阳性牛、淋巴肉瘤牛被毛中Mn含量依次显著降低(P<0.05),与SCE呈显著性负相关(r=-0.969);Co含量依次增高,与SCE呈正相关(r=0.814);Cu/Zn值与SCE呈正相关(r=0.783)。用泰氏锥虫间接红细胞凝集试验(IHA)检测泰氏锥虫感染情况,发现血检和MID双阳性中、MID单阳性牛以及健康对照牛IHA阳性率分别为86.18%,4(?).45%,和31.25%,而淋巴肉瘤牛均为阳性,与SCE呈正相关(r=0.753),具有显著性意义(P<0.05)。
Sister chromatid exchange (SCE) frequency was observed in 64 dairy cows using short-term culture of peripheral blood lymphocytes. The results showed that lymphosarcoma cattle, blood tests and MID double positive cattle, MID single positive cattle and healthy control cattle SCE / Cell were 9.36 ± 0.349 (S · E), 6.60 ± 0.495, 4.89 ± 0.379 and 3.59 ± 0.328, respectively. There was a significant difference between the groups (P <0.01). In the meantime, 10 elements of Zn, Ni, Mn, Cu, Co, Pb, Mo, Cr, Ba and B were measured by plasma source emission spectroscopy. There was a significant negative correlation between the content of Mn and the content of Co (r = -0.969), the content of Co in turn increased, which was positively correlated with SCE (r = 0.814) There was a positive correlation with SCE (r = 0.783). The detection of Trypanosoma tenella infection by Trypanna reicularis indirect hemagglutination test (IHA) showed that the positive rates of IHA in MID and MID were 86.18% and 4 (?), Respectively. 45%, and 31.25%, respectively. However, the positive rate of lymphosarcoma was positively correlated with SCE (r = 0.753), with significant significance (P <0.05).