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为了探讨嫁接烟草响应干旱胁迫的生理和分子机制,以耐旱品种龙江851为砧木,敏感品种金星6007为接穗,得到自嫁接组合JX6007/JX6007和嫁接组合JX6007/LJ851。采用盆栽试验研究了干旱对两种组合的烟草单株生物量、叶片抗氧化酶活性、活性氧以及膜脂过氧化的影响。结果表明:干旱胁迫条件下,JX6007/LJ851烟株的生物量、叶片叶绿素含量(质量分数)以及抗氧化酶活性均不同程度高于JX6007/JX6007,活性氧含量以及膜脂过氧化程度均低于JX6007/JX6007。荧光定量PCR分析显示,嫁接显著提高了胁迫响应基因LEA5、AREB、CDPK2和ERD10C的表达量。因此,以耐旱品种作砧木的嫁接烟株可以通过调节抗氧化酶活性和胁迫响应基因的表达来提高其抗旱性。
In order to investigate the physiological and molecular mechanisms of grafted tobacco in response to drought stress, a drought tolerant variety, Longjiang 851, was used as rootstock and the susceptible cultivar Venus 6007 was used as a scion to obtain self-grafting combination JX6007 / JX6007 and grafting combination JX6007 / LJ851. Pot experiments were conducted to study the effects of drought on the biomass per plant, antioxidant enzyme activity, active oxygen and membrane lipid peroxidation in two combinations. The results showed that under drought stress, the biomass, leaf chlorophyll content and antioxidant enzyme activity of JX6007 / LJ851 tobacco plants were all higher than JX6007 / JX6007, the content of reactive oxygen species and membrane lipid peroxidation were lower than JX6007 / JX6007. Fluorescent quantitative PCR analysis showed that grafting significantly increased the expression of stress response genes LEA5, AREB, CDPK2 and ERD10C. Therefore, grafted tobacco plants with drought-tolerant cultivars as rootstocks could improve their drought tolerance by regulating the expression of antioxidant enzyme activity and stress-responsive genes.