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观察了犬冠脉内灌注Ｎ－单甲基左旋精氨酸（Ｌ－ＮＭＭＡ）后再灌注Ｌ－精氨酸（Ｌ－Ａｒｇ）和单独灌注Ｌ－ＮＭＭＡ前后冠脉血流动力学、冠脉血流储备以及冠脉对不同浓度的乙酰胆碱（Ａｃｈ）反应的变化，同时用放免法测定冠脉前降支（ＬＡＤ）伴行静脉血中内皮素－１（ＥＴ－１）含量。结果发现，Ｌ－Ａｒｇ完全逆转了灌注Ｌ－ＮＭＭＡ引起的血流动力学改变，使心率回升，下降的基础冠脉流量（ＣＢＦ），从２０±８ｍｌ／ｍｉｎ回升至２８±７ｍｌ／ｍｉｎ，Ｐ＜０．０５），降低的冠脉储备恢复，从５１±１０ｍｌ／ｍｉｎ升至９４±１５ｍｌ／ｍｉｎ，Ｐ〈０．０１），ＥＴ－１的含量不再升高，从１５．５±３．０ｎｇ／Ｌ下降至５．０±２．０ｎｇ／Ｌ，Ｐ〈０．０１），Ａｃｈ介导的ＣＢＦ增加不再受到抑制（Ｐ〈０．０１）。结果提示提供外源性Ｌ－Ａｒｇ可增加一氧化氮（ＮＯ）的产生，使由于ＮＯ抑制而产生的血流动力学改变和ＥＴ－１升高发生逆转。
Coronary hemodynamics and coronary arteries were observed before and after coronary perfusion with L-arginine (L-Arg) and L-NMMA alone Blood flow reserve and the change of coronary response to different concentrations of acetylcholine (Ach) were determined. The content of endothelin-1 (ET-1) in venous blood of anterior descending coronary artery (LAD) was measured by radioimmunoassay. The results showed that L-Arg completely reversed the hemodynamic changes caused by perfusion of L-NMMA. The basal coronary flow (CBF), the heart rate rebounded and decreased from 20 ± 8ml / min to 28 ± 7ml / min, P <0.05), reduced coronary reserve recovery from 51 ± 10 ml / min to 94 ± 15 ml / min, P <0.01), and ET-1 content no longer increased from 15.5 ± 3 .0ng / L to 5.0 ± 2.0ng / L, P <0.01). Ach-mediated increase of CBF was no longer inhibited (P <0.01). The results suggest that providing exogenous L-Arg increases the production of nitric oxide (NO), reversing the hemodynamic changes and ET-1 elevation due to NO inhibition.