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目的:建立HPLC法同时测定漏芦中咖啡酸、甘草苷、迷迭香酸、齐墩果酸、β-谷甾醇、豆甾醇的含量。方法:采用Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm)色谱柱;流动相为甲醇-0.04%磷酸,梯度洗脱;流速1.0m L/min;检测波长220 nm;柱温30℃。结果:咖啡酸、甘草苷、迷迭香酸、齐墩果酸、β-谷甾醇、豆甾醇分别在0.52~104.12μg/m L、0.51~101.23μg/m L、1.01~201.31μg/m L、0.52~103.21μg/m L、0.51~100.26μg/m L、0.52~103.67μg/m L范围内与相应的峰面积呈良好的线性关系,平均加样回收率的RSD分别为0.77%、1.50%、0.75%、1.82%、1.89%、2.02%。结论:该方法能准确、快速分析漏芦中6个活性成分,灵敏度高,重现性好,为质量评价奠定了基础。
Objective: To establish a HPLC method for the simultaneous determination of caffeic acid, liquiritin, rosmarinic acid, oleanolic acid, β-sitosterol and stigmasterol in Loulu. Method: Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol-0.04% phosphoric acid with gradient elution. The flow rate was 1.0 m L / min. Results: The concentrations of caffeic acid, glycyrrhizin, rosmarinic acid, oleanolic acid, β-sitosterol and stigmasterol were respectively in the range of 0.52 ~ 104.12μg / mL, 0.51 ~ 101.23μg / mL and 1.01 ~ 201.31μg / mL , 0.52 ~ 103.21μg / m L, 0.51 ~ 100.26μg / m L and 0.52 ~ 103.67μg / m L, respectively. The average RSDs were 0.77% and 1.50 %, 0.75%, 1.82%, 1.89%, 2.02%. Conclusion: The method can accurately and rapidly analyze six active ingredients in Leuciscus with high sensitivity and reproducibility, which lays the foundation for the quality evaluation.