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目的探讨广州长春花中文多灵生物碱在一年内的变化规律,为长春花GAP种植提供依据。方法采用Yilite C18柱(250 mm×4.6 mm,10μm),流动相为甲醇-水(62∶38,二乙胺调pH11),流速1 mL.min-1,检测波长254 nm。结果采用HPLC测定了广州长春花不同样品中文多灵生物碱的含量。结论一年的生长时期中,广州长春花中文多灵生物碱的含量差异较大。茎中文多灵的含量在10～2月处于上升阶段,2～9月总体处于下降阶段。叶中文多灵的含量则在10～3月与4～9月,分别呈现上升与下降的趋势。花与果中文多灵生物碱的含量动态变化与茎、叶相比,较为复杂。茎、叶、花、果中文多灵的含量分别在2月、3月、6月及4月达到最大值。
Objective To investigate the change rule of Polygonatum alkaloid in Guangzhou Prunella vulgaris during one year and provide the basis for the cultivation of Catharanthus roseus in GAP. Methods Yilite C18 column (250 mm × 4.6 mm, 10 μm) was used. The mobile phase consisted of methanol-water (62:38, diethylamine adjusted to pH 11) and the flow rate was 1 mL.min-1. The detection wavelength was 254 nm. Results HPLC was used to determine the content of alkaloids in different samples of P. vinca from Guangzhou. Conclusions In the growth period of one year, the content of Polyindole alkaloids in Guangzhou Changchun flower is quite different. Stem Chinese content of more spiritual in 10 ~ February in the rising stage, 2 to 9 months in general decline. More Chinese leaf content in the spirit of 10 to March and 4 to 9 months, respectively, showing the trend of rising and falling. Flower and fruit Chinese multi-ling alkaloids dynamic changes compared with stems and leaves, more complex. The contents of stem, leaf, flower and fruit polypore reached the maximum in February, March, June and April respectively.