逆转录和聚合酶链反应的结合(RT/PCR)对于检测丙型肝炎病毒(HCV)感染患者血清中的 HCV RNA 是非常有用的工具。然而由于PCR 的高度敏感性,用于研究肝等组织中 HCV的存在时,难以区分病毒核酸是来自肝细胞内,还是来自可能污染肝标本的血液。鉴于 HCV 是与黄病毒有远缘的单股正链 RNA 病毒,推测在 The combination of reverse transcription and polymerase chain reaction (RT / PCR) is a very useful tool for the detection of HCV RNA in the serum of patients infected with hepatitis C virus (HCV). However, due to the high sensitivity of PCR for studying the presence of HCV in tissues such as liver, it is difficult to distinguish whether the viral nucleic acid is from hepatocytes or from blood that may contaminate liver specimens. Given that HCV is a single-stranded positive-strand RNA virus that is distant from the flavivirus, speculation exists