最新研究表明MicroRNAs(miRNA)在细胞内的异常表达与人类疾病具有密切的关系,并且miRNA有望成为一种肿瘤标志物和治疗靶点。miRNA在细胞增殖、分化和凋亡等生物学过程中起着重要作用。最新研究表明miRNA在细胞内的异常表达与人类疾病具有密切的关系,并且miRNA有望成为一种肿瘤标志物和治疗靶点。传统的Northern杂交法和实时定量PCR技术常被用于miRNA的分析,但其灵敏度低与特异性差等局限性限制了这两种方法的广泛应用。因 Recent studies show that the abnormal expression of MicroRNAs (miRNAs) in cells is closely related to human diseases, and miRNAs are expected to become a tumor marker and therapeutic target. MiRNAs play an important role in the biological processes such as cell proliferation, differentiation and apoptosis. Recent studies show that abnormal expression of miRNAs in cells is closely related to human diseases, and miRNAs are expected to become a tumor marker and therapeutic target. Traditional Northern hybridization and real-time quantitative PCR are often used for the analysis of miRNAs, but their low sensitivity and poor specificity limit the widespread use of these two methods. because