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高压诱导蛋白质发生构象变化,引起蛋白质去折叠,并在某种条件下发生聚集,其结果是蛋白质原有的构造被破坏、发生变性。这些过程与蛋白质本身的结构密切相关。同时,折叠中间体结构的揭示对于研究蛋白质去折叠过程也具有重要意义。本文综述了高压诱导下多种谱学方法用于蛋白质构象变化的研究进展,主要包括高压红外光谱、高压荧光光谱及高压小角X射线扫描等多种技术手段在研究中蛋白质构象变化方面所能提供的重要信息,并对相关领域存在的问题及今后的发展作出展望。
High pressure induces a conformational change in the protein, causing the protein to de-fold and aggregate under certain conditions, with the result that the original protein structure is disrupted and denatured. These processes are closely related to the structure of the protein itself. At the same time, revealing the structure of folded intermediates is also of great significance for studying the process of protein unfolding. This review summarizes the research advances in the conformational changes of proteins under high pressure-induced multiple spectroscopy, including high-pressure infrared spectroscopy, high-pressure fluorescence spectroscopy and high-pressure small-angle X-ray scanning and other techniques can provide the conformational changes in the study The important information, and the problems in related fields and future development prospects.