论文部分内容阅读
目的:用整体动物和离体海马脑片实验研究复方人参制剂的抗脑缺氧作用.方法:(1)在密闭三角烧瓶中观察小白鼠缺氧存活时间;(2)在大鼠离体海马脑片上观察缺氧过程中缺氧损伤电位(hypoxic injury potential,HIP)的出现率及缺氧后诱发顺向群峰电位(orthodromic population spike,OPS)的恢复率和恢复程度.结果:(1)小白鼠缺氧存活时间(min):正常对照组26.44±4.58,用药组48.46±16.80,用药组显著延长(P<0.001);(2)对照组OPS恢复率20%,恢复程度30.2±36.6%,HIP发生率80%;人参制剂组OPS恢复率100%,恢复程度84.90±35.0%,未出现HIP.与对照组比较,OPS恢复显著,HIP发生率显著降低(P<0.05).结论:实验用复方人参制剂有明显的抗脑缺氧作用,其作用机制可能是人参降低神经元的兴奋性,减少能耗,从而减轻脑损伤.
OBJECTIVE: To study the anti-cerebral anoxia effect of compound ginseng preparations using whole animal and isolated hippocampal slices. Methods: (1) Observe the hypoxic survival time of mice in sealed conical flask; (2) In vitro rat hippocampus The rate of occurrence of hypoxic injury potential (HIP) during hypoxia and the recovery rate and recovery degree of orthodromic population spike (OPS) after hypoxia were observed on brain slices. Results: (1) Hypoxia survival time (min) of mice: normal control group 26.44±4.58, medication group 48.46±16.80, medication group significantly prolonged (P<0.001); (2) control group OPS recovery rate 20%, degree of recovery 30.2±36.6% The incidence of HIP was 80%. The recovery rate of OPS in the ginseng preparation group was 100%, and the degree of recovery was 84.90±35.0%. HIP did not appear. Compared with the control group, the OPS recovery was significant, and the HIP incidence was significantly lower (P<0.05). Conclusion: Experiments Compound ginseng preparations have obvious anti-cerebral anoxia effect, and its mechanism of action may be that ginseng reduces neuronal excitability and reduces energy consumption, thereby reducing brain damage.