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板栗疫病菌(Cryphonectria parasitica)是引起板栗疫病的病原真菌。低毒病毒是一类侵染板栗疫病菌的无衣壳正链RNA病毒。无病毒栗疫病菌野生型菌株在PDA培养基上形成桔黄色菌落,而带毒株在则形成白色菌落。本研究利用已知的不同机制的抗病毒药物处理无病毒和感染病毒的板栗疫病菌菌株,观察菌丝体颜色变化并检测菌丝体中病毒双链RNA(dsRNA)的含量。结果显示,抗病毒药物处理后,带毒株系EP721和Euro7的菌丝体颜色表型发生明显变化,且病毒dsRNA累积量与菌丝体颜色变化存在明显相关性:随着药物浓度增大,菌丝体颜色加深,病毒dsRNA积累量下降。因此,可以根据菌株颜色的变化判断药物对病毒复制或症状表现是否有效,从而极大简化抗RNA病毒药物的初步筛选过程。
Cryphonectria parasitica is a pathogenic fungus that causes chestnut blight. Hypox virus is a type of capsid-positive-strand RNA virus that infects the chestnut blight. Wild-type strains of Cryphonectria eutrophus form orange-colored colonies on PDA medium, while the infected strains form white colonies. In this study, virus-free and virus-infected C. bryogenes strains were treated with known antiviral agents with different mechanisms to observe the color change of mycelium and to detect the content of double-stranded RNA (dsRNA) in mycelium. The results showed that the mycelial color phenotypes of EP721 and Euro7 were significantly changed after antiviral treatment, and the accumulation of virus dsRNA was significantly correlated with the color change of mycelium: With the increase of drug concentration, Mycelium color deepened, the virus dsRNA accumulation decreased. Therefore, it is possible to judge whether the drug is effective for virus replication or symptom presentation according to the change of the color of the strain, thereby greatly simplifying the preliminary screening process of the anti-RNA viral drug.