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利用 RT- PCR方法 ,从胎儿脾脏和肝脏组织中克隆出一个 342 bp的基因片段 ,并测定了其核苷酸序列。在基因库中未见相同序列报道 ,但该片段与 p62 dok- PTB结构域核苷酸序列有 93%的同源性 ,提示它是一种新的 p62 dok- PTB结构域样基因片段。
A 342 bp gene fragment was cloned from fetal spleen and liver tissue by RT-PCR and the nucleotide sequence was determined. The same sequence was not found in the gene pool, but the fragment shared 93% homology with the p62 dok-PTB domain, suggesting that it is a novel p62 dok-PTB domain-like gene fragment.