Rapid screening and distribution of bioactive compounds in different parts of Berberis petiolaris us

来源 :Journal of Pharmaceutical Analysis | 被引量 : 0次 | 上传用户:mynameiscaohaoxiang
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Berberis petiolaris Wall,ex G.Don,an unexplored medicinal plant belonging to the family Berberidaceae,is a large deciduous shrub found in Western Himalaya between 1800-3000 m.Chemical profiling of fruit,leaf,root and stem was done by direct analysis in real time mass spectrometry followed by multivariate analysis for discrimination among the plant parts.The bioactive compounds,including magnoflorine,berberine,jatrorrhizine,thalifendine/berberrubine,demethyleneberberine,reticuline,8-oxoberberine,N-methyltetrahydroberberine,tetrahydropalmatine,tetrahydroberberine and palmatine,were identified by their exact mass measurement and the corresponding molecular formula of each compound.A comparative study of distribution pattern for all these bioactive alkaloids showed qualitative and quantitative variations in different parts of B.petiolaris.Principal component analysis clearly discriminated each part of B.petiolaris plant. Berberis petiolaris Wall, ex G. Don, an unexplored medicinal plant belonging to the family Berberidaceae, is a large deciduous shrub found in Western Himalaya between 1800-3000 m. Chemical profiling of fruit, leaf, root and stem was done by direct analysis in real time mass spectrometry followed by multivariate analysis for discrimination among the bioactive compounds, including magnoflorine, berberine, jatrorrhizine, thalifendine / berberrubine, demethyleneberberine, reticuline, 8- oxoberberine, N- methyltetrahydroberberine, tetrahydropalmatine, tetrahydroberberine and palmatine, by their exact mass measurement and the corresponding molecular formula of each compound. A comparative study of distribution pattern for all these bioactive alkaloids showed qualitative and quantitative variations in different parts of B. petiolaris. Principle component identification every discriminated each part of B. petiolaris plant .
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