建立了新疆棉籽油脱臭馏出物中α-生育酚的HPLC分析方法。选用Symmetry C18色谱柱,以甲醇-乙腈(50:50v/v)为流动相,在292nm波长进行检测,柱温20℃,流速为1 mL/min,获得了较理想的分离效果。该方法线性范围为4.39～439.00μg/mL,平均回收率为90.48%～99.59%(RSD=3.62%)。信噪比为3时的最低检测限为12.69 ng,重复性好,准确可靠,是棉籽油脱臭馏出物中α-生育酚测定的较理想的方法。 A HPLC method for the determination of α-tocopherol in Xinjiang cottonseed oil deodorizer distillate was established. Symmetry C18 column was used with methanol-acetonitrile (50:50 v / v) as the mobile phase and detected at 292 nm with a column temperature of 20 ℃ and a flow rate of 1 mL / min. The optimal separation was obtained. The linear range of this method was 4.39 ~ 439.00μg / mL. The average recoveries ranged from 90.48% to 99.59% (RSD = 3.62%). The minimum detection limit of 12.69 ng with signal-to-noise ratio of 3 is good, repeatability, accurate and reliable, which is an ideal method for determination of α-tocopherol in cottonseed oil deodorizer distillate.