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背景:丹参可有效减轻卡那霉素对CBA小鼠的耳毒性效应;然而,其是否也能防护庆大霉素对豚鼠的耳蜗毒性,目前尚不明确。目的:研究丹参注射液对庆大霉素耳蜗毒性的防护作用。设计:完全随机对照的实验研究。地点和对象:实验在中国医科大学听力研究室进行。选用清洁级健康纯白红目豚鼠40只,雌雄不限,体质量250～300g,耳郭反射灵敏,鼓膜及外耳道正常,由中国医科大学第二医院动物中心提供。干预:动物随机分成4组,即对照组、庆大霉素组、庆大霉素+丹参注射液组和丹参注射液组,每组各10只。庆大霉素组每日腹腔注射硫酸庆大霉素100mg/kg;丹参注射液组每日腹腔注射丹参注射液6g/kg;庆大霉素+丹参注射液组同时注射硫酸庆大霉素100mg/kg和丹参注射液6g/kg;对照组则每日腹腔注射等量生理盐水。4组皆连续用药10d。用药期间每天监测体质量以调整药量。全部实验由作者和另一名硕士研究生完成。主要观察指标:应用听脑干反应(auditorybrainstemresponse,ABR)测试,观察用药前后听阈变化。应用透射及扫描电镜技术,观察豚鼠耳蜗形态学变化。结果:用药10d后,庆大霉素组ABR阈值(36.00±5.48)dBSPL明显升高,与对照组(17.00±4.47)dBSPL比较差异有显著性意义(t=6.008,P<0.01);庆大霉素+丹参注射液组ABR阈值27.00±4.47)dBSPL较(庆大
BACKGROUND: Radix Salviae Miltiorrhizae can effectively reduce the ototoxic effect of kanamycin on CBA mice; however, it is still not clear whether it can also protect the cochlear toxicity of gentamicin against guinea pigs. Objective: To study the protective effect of Danshen injection on gentamicin cochlear toxicity. Design: A fully randomized controlled experimental study. Location and Subjects: Experiments were conducted at the Hearing Laboratory, China Medical University. Selection of clean grade healthy white red guinea pigs 40, male or female, body weight 250 ~ 300g, agar reflection sensitive, normal tympanic membrane and external auditory canal, provided by the Animal Center of the Second Hospital of China Medical University. Intervention: The animals were randomly divided into 4 groups: control group, gentamicin group, gentamicin + Danshen injection group and Danshen injection group, with 10 rats in each group. The gentamicin group was intraperitoneally injected with 100 mg / kg gentamicin sulfate per day; the Salvia miltiorrhiza injection group was intraperitoneal injected with 6 g / kg Salvia miltiorrhiza injection; the gentamicin plus salvia miltiorrhiza injection group was simultaneously injected with 100 mg gentamicin sulfate / kg and Salvia injection 6g / kg; control group were injected intraperitoneally with the same amount of saline. 4 groups were continuous medication 10d. Daily monitoring of body weight during medication to adjust the dose. All experiments were done by the author and another master graduate student. MAIN OUTCOME MEASURES: Auditory brainstem response (ABR) was used to observe the change of hearing threshold before and after treatment. Transmission and scanning electron microscopy techniques were used to observe morphological changes of cochlea in guinea pigs. Results: After 10 days of treatment, the ABSP threshold (36.00 ± 5.48) dBSPL in gentamicin group was significantly higher than that in control group (17.00 ± 4.47) dBSPL (t = 6.008, P <0.01) ADM + Danshen injection ABR threshold 27.00 ± 4.47) dBSPL (Qingda