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Background: Heat shock proteins (HSPs) are a family of highly conserv ed prote ins found ubiquitously in mammalian cells, believed to be regulators of normal c ell physiology and the cellular stress response. In addition, the small 27- kDa heat shock protein (HSP27) has previously been found to be a differentiation ma rker for keratinocytes and a prognostic marker associated with increased surviva l in certain cancerous tumors. Methods: Using immunohistochemistry on routinely processed paraffin sections, we examined skin biopsies from 15 invasive melanoma s, 13 intradermal nevi, and two compound nevi immunostained with a mouse monoclo nal antibody to HSP27. In addition, cultured melanocytes were heat stressed at 4 5° C for 1 h and then fixed and immunostained in order to localize HSP27 expres sion intracellularly. Results: We found cytoplasmic and strong perinuclear stain ing of HSP27 in melanocytes in normal skin, in melanomas, and in nevi. Nuclear r eactivity was absent. In addition, in cultured non-malignant melanocytes, HSP2 7 expression relocated from the cytoplasm to the nucleus with heat stress. Concl usions: To our knowledge, this investigation is the first to demonstrate that HS P27 is expressed in melanocytes in normal skin, in nevi, and in non-malignant cultured melanocytes.
Background: Heat shock proteins (HSPs) are a family of highly conserv ed prote ins found ubiquitously in mammalian cells, believed to be regulators of normal c ell physiology and the cellular stress response. In addition, the small 27-kDa heat shock protein HSP27) has previously been found to be differentiating ma rkers for keratinocytes and a prognostic marker associated with increased survivin in certain cancerous tumors. Methods: Using immunohistochemistry on routinely processed paraffin sections, we examined skin biopsies from 15 invasive melanoma s, 13 intradermal nevi, and two compound nevi immunostained with a mouse monoclo nal antibody to HSP27. In addition, cultured melanocytes were heat stressed at 4 5 ° C for 1 h and then fixed and immunostained in order to localize HSP27 expres sion intracellularly. cytoplasmic and strong perinuclear stain ing of HSP27 in melanocytes in normal skin, in melanomas, and in nevi. Nuclear r eactivity was absent. , in cultured non-malignant melanocytes, HSP2 7 relocated from the cytoplasm to the nucleus with heat stress. Concl usions: To our knowledge, this investigation is the first to demonstrate that HS P27 is expressed in melanocytes in normal skin, in nevi, and in non-malignant cultured melanocytes.